An acid phosphatase locus expressed in mouse kidney (Apk) and its genetic location on chromosome 10 |
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Authors: | James E. Womack Sandra Belsky Auerbach |
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Affiliation: | (1) The Jackson Laboratory, 04609 Bar Harbor, Maine;(2) Present address: The Institute of Comparative Medicine, College of Veterinary Medicine, Texas A&M University, 77843 College Station, Texas |
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Abstract: | A genetic locus controlling the electrophoretic mobility of an acid phosphatase in mouse kidney is described. This locus, called acid phosphatase-kidney (Apk), is not expressed in erythrocytes, liver, spleen, heart, lung, brain, skeletal muscle, stomach, or testes. The product of Apk hydrolyzes the substrate naphthol AS-MX phosphoric acid but is not active on a-naphthylphosphate or 4-methylumbelliferylphosphate. It is not inactivated by 50 C for 1 hr, nor is its electrophoretic mobility altered by incubation with neuraminidase. The locus is invariant among 31 inbred strains (Apka), with a variant allele (Apkm) observed only in Mus musculus molossinus. Codominant expression was observed in F1 hybrids of M. m. molossinus and inbred strains. Apk was mapped on Chr 10, near the neurological mutant waltzer (v).This work was supported by Contract NO1-ES42159 from the National Institute of Environmental Health Sciences and by Grant 1-476 from the National Foundation—March of Dimes. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care. |
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Keywords: | mouse acid phosphatase Apk, chromosome 10 lysosomal enzymes isozymes allozymes |
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