The fusogenic state of mayaro virus induced by low pH and by hydrostatic pressure |
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Authors: | Mônica S Freitas Andrea T Da Poian Ortrud M Barth Moacyr A Rebello Jerson L Silva Luciane P Gaspar |
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Institution: | Programa de Biologia Estrutural, Instituto de Bioquímica Médica, Centro Nacional de Ressonancia Magnêtica Nuclear de Macromolêculas Jiri Jonas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. |
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Abstract: | Mayaro virus is an enveloped virus that belongs to the Alphavirus genus. To gain insight into the mechanism involved in Mayaro virus membrane fusion, we used hydrostatic pressure and low
pH to isolate a fusion-active state of Mayaro glycoproteins. In response to pressure, E1 glycoprotein undergoes structural
changes resulting in the formation of a stable conformation. This state was characterized and correlated to that induced by
low pH as measured by intrinsic fluorescence, 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt fluorescence,
fluorescence resonance energy transfer, electron microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
In parallel, we used a neutralization assay to show that Mayaro virus in the fusogenic state retained most of the original
immunogenic properties and could elicit high titers of neutralizing antibodies. |
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Keywords: | Mayaro virus fusogenic state hydrostatic pressure low pH inactivation |
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