Effects of lithium chloride as a potential radioprotective agent on radiation response of DNA synthesis in mouse germinal cells |
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Authors: | D Bhattacharjee R Rajan L Krishnamoorthy B B Singh |
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Institution: | (1) Radiation Biology & Biochemistry Division, Bhabha Atomic Research Centre, Mumbai 400 085, India, IN;(2) Kidwai Memorial Institute of Oncology, Bangalore 560 029, India, IN |
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Abstract: | Mouse spermatogonial germ cells are highly sensitive to ionizing radiation. Lithium salts are reported to stimulate the postirradiation
recovery of hematopoietic marrow cells. We have, therefore, examined whether administered lithium chloride (LiCl) would also
be able to protect the mouse germinal cells against radiation injury. Taking DNA synthesis as an endpoint, our results show
that the testicular DNA-specific activity in irradiated mice was higher by 61% on average when they had been pretreated with
LiCl both 24 h and 1 h prior to γ-irradiation (2.0 Gy). It was also observed that the DNA synthetic activity in the germinal cells fully recovered after LiCl
pretreatment at doses of 40 mg per kg body weight prior to total body irradiation of 0.05–0.25 Gy, whereas at doses of 0.5–6.0
Gy, following the same procedure of LiCl pretreatment, only an incomplete recovery was observed. The dose reduction factor
for LiCl is 1.84. The current findings indicate that pretreatment with LiCl provides considerable protection against radiation
damage in mouse spermatogonia.
Received: 18 October 1996 / Accepted in revised form: 3 April 1997 |
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