Single-cell-bioreactors as end of miniaturization approaches in biotechnology: progresses with characterised bioreactors and a glance into the future |
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Authors: | Stephan Michael Meier Holger Huebner Rainer Buchholz |
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Institution: | (1) Institute of Bioprocess Engineering, University of Erlangen-Nuremberg, Erlangen, Germany |
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Abstract: | Incidents with single cells and their genesis have not been the major focus of science up to now. This fact is supported by
the difficulties one faces when wanting to monitor and cultivate small populations of cells in a defined compartment under
controlled conditions, in vitro. Several approaches of up- and down-scaling have often led to poorly understood results which
might be better elucidated by understanding the cellular genesis as a function of its microenvironment. This review of the
approaches of scale-up and scale-down processes illustrates technical possibilities and shows up their limitations with regard
to obtainable data for the characterisation of cellular genesis and impact of the cellular microenvironment. For example,
stem cell research advances underline the lack of information about the impact of the microenvironment on cellular development.
Finally, a proposal of future research efforts is given on how to overcome this lack of data via a novel bioreactor setup. |
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Keywords: | Utem cells Single cell bioreactor Immobilisation Scale down Scale-up |
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