Synthesis of oligosaccharides using immobilised 1,2-a-mannosidase from Aspergillus phoenicis: Immobilisation-dependent modulation of product spectrum |
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Authors: | Sony Suwasono Robert A Rastall |
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Institution: | (1) Biotechnology and Biochemical Engineering Group, Department of Food science and Technology, University of Reading, P.O. Box 226, Reading, UK |
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Abstract: | A specific 1,2-a-mannosidase from A. phoenicis was immobilised on China clay, cellulose DE-52 and by entrapment in sodium alginate beads with respective yields of 19%, 16% and 18% in synthesis reactions consisting of 80% (w/w) mannose. Mana1 6man was the predominant product with China clay- and DE-52-immobilised enzymes with lesser amounts of mana1 2 and mana1 3. With the alginate bead-immobilised enzyme, mana1 2 was the predominant linkage formed. |
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