Feeding strategies for E. coli fermentations demanding an enriched environment |
| |
Authors: | L. de Maré C. Cimander A. Elfwing P. Hagander |
| |
Affiliation: | (1) Department of Automatic Control, LTH, Lund University, Box 118, 221 00 Lund, Sweden;(2) Novozymes Biopharma AB, Lund, Sweden |
| |
Abstract: | The addition of a carbon nutrient feed to a fed-batch cultivation is often not enough to obtain satisfactory growth and/or production. In some cases, an additional feed with for example supplementary amino acids or complex media is required. This work presents the development of feeding strategies where more than one feed is required and the knowledge of the growth requirements is low. Simulations and cultivations with E. coli are shown using the proposed feed controllers which are based on a probing control concept. The strategies work well and they can be used to shorten the process development phase considerably. |
| |
Keywords: | Fermentation process Feeding strategy Fed-batch cultivation Escherichia coli Complex medium Amino acid |
本文献已被 PubMed SpringerLink 等数据库收录! |