Developmental changes in gangliosides during myogenesis of a rat myoblast cell line and its drug resistant variants. |
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| Authors: | R Whatley S K Ng J Rogers W C McMurray B D Sanwal |
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| Institution: | Department of Biochemistry, University of Western Ontario, London, Ontario, Canada, N6A 5C1 |
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| Abstract: | Cloned cells of a myoblast line show the presence of GM3, GM2, GM1 and GD1a gangliosides. The amount of GM3, GM2 and GM1 gangliosides does not vary significantly during the differentiation of myoblasts to myotubes. However, the concentration of GD1a transiently increases almost 3-fold just prior to the fusion of myoblasts and returns to the basal levels in the myotubes. Mutant myoblasts selected for 5-azacytidine resistance and unable to fuse produce only GM3 and traces of GM2. We conclude that GD1a probably participates in the fusion process through yet unknown mechanism. |
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| Keywords: | GLC gas-liquid chromatography HGPRTase hypoxanthine-guanine phosphoribosyl transferase SDS sodium dodecyl sulfate TLC thin layer chromatography |
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