Enhanced encapsulation of chondrocytes within a chitosan/hyaluronic acid hydrogel: a new technique |
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Authors: | Sowmya Ramesh Karthikeyan Rajagopal Dhanesh Vaikkath Prabha D Nair Vrisha Madhuri |
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Institution: | 1. Paediatric Orthopaedics Unit, Department of Orthopaedics, Christian Medical College Vellore, Vellore, 632004, Tamil Nadu, India 2. Division of Tissue Engineering and Regeneration Technologies, BMT Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 695012, India
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Abstract: | Two encapsulation techniques for rabbit chondrocytes in chitosan/hyaluronic acid gel have been compared. The standard technique involves the cross-linking of chitosan and hyaluronic acid at 2:1 (w/w). In the modified technique, cells were initially added to 33 % of hyaluronic acid dialdehyde and the gelation process was completed with the remaining 67 %. This minimised the cell loss and improved the encapsulation of the cells. By the third week, the modified technique showed better seeding density, with matrix synthesis (per scaffold) of 11 μg as compared to 1.1 μg in the current technique. Relative expression of collagen II with the current technique and the modified technique were 6.4 % and ~1,600 % respectively. The modified technique was superior for matrix synthesis and maintenance of phenotype. |
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