Chloroplast molecular farming: efficient production of a thermostable xylanase by Nicotiana tabacum plants and long-term conservation of the recombinant enzyme |
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Authors: | Laura Pantaleoni Paolo Longoni Lorenzo Ferroni Costanza Baldisserotto Sadhu Leelavathi Vanga Siva Reddy Simonetta Pancaldi Rino Cella |
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Affiliation: | 1. Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9, 27100, Pavia, Italy 4. Department of Botany and Plant Biology, University of Geneva, Sciences III, Quai E. Ansermet30, 1211, Geneve 4, Switzerland 2. Department of Life Sciences and Biotechnologies, University of Ferrara, C.so Ercole I d’Este 32, 44121, Ferrara, Italy 3. Plant Transformation Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India
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Abstract: | The high cost of recombinant enzymes for the production of biofuel from ligno-cellulosic biomass is a crucial factor affecting the economic sustainability of the process. The use of plants as biofactories for the production of the suitable recombinant enzymes might be an alternative to microbial fermentation. In the case of enzyme accumulation in chloroplasts, it is fundamental to focus on the issue of full photosynthetic efficiency of transplastomic plants in the field where they might be exposed to abiotic stress such as high light intensity and high temperature. Xylanases (EC 3.2.1.8), a group of enzymes that hydrolyse linear polysaccharides of beta-1,4-xylan into xylose, find an application in the biofuel industry favouring biomass saccharification along with other cell-wall degrading enzymes. In the present study, we analysed how a high level of accumulation of a thermostable xylanase in tobacco chloroplasts does not impact on photosynthetic performance of transplastomic plants grown outdoors. The recombinant enzyme was found to be stable during plant development, ex planta and after long-term storage. |
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