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A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells |
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| Authors: | Ailing Teo Athanasios Mantalaris Kedong Song Mayasari Lim |
| Institution: | 1. School of Chemical & Biomedical Engineering, Nanyang Technological University, Singapore, Singapore 2. Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, UK 3. School of Chemical Engineering & Technology, Dalian University of Technology, Dalian, China 4. Division of Bioengineering, Nanyang Technological University, Block N1.3, 70 Nanyang Drive, Singapore, 637457, Singapore |
| Abstract: | Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 105 cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs. |
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