The development of transgenic mice for the expression of large amounts of human lysozyme in milk |
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Authors: | Xiaojie Wu Yanli Lin Yongyi Xi Zhenlu Shao Yanrong Zhou Fang Liu Hongxing Chen |
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Institution: | 1. Cell Engineering Department, Beijing Institute of Biotechnology, No. 20 Dongdajie Street, Beijing, 100071, People’s Republic of China
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Abstract: | Human lysozyme (hLYZ) has important potential applications as antimicrobial medicine and food additive. To develop a robust expression vector that ensures expression of large amounts of hLYZ in milk, here a 26,267 bp chimeric mouse whey acidic protein (mWAP)::hLYZ cassette was constructed and used as a mammary gland-specific expression vector, in which a 3,010 bp genomic sequence in the 24,466 bp mWAP gene locus was substituted by a 4,811 bp genomic sequence of hLYZ, exactly from the start codon to the stop codon. Corresponding transgenic mice were generated, and enzymatically-active hLYZ was expressed at 18.4–35 g l?1 in the milk of most transgenic mouse lines. Our transgenic mice carrying chimeric mWAP::hLYZ represent a model system for cost-effective production of hLYZ. |
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