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Lectin of Abelmoschus esculentus (okra) promotes selective antitumor effects in human breast cancer cells |
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| Authors: | Leonardo G Monte Tatiane Santi-Gadelha Larissa B Reis Elizandra Braganhol Rafael F Prietsch Odir A Dellagostin Rodrigo Rodrigues e Lacerda Carlos A A Gadelha Fabricio R Conceição Luciano S Pinto |
| Institution: | 1. Laboratório de Biotecnologia Vegetal e Prote?mica, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, P.O. Box 354, Pelotas, RS, 96010-900, Brazil 4. Departamento de Biologia Molecular, Universidade Federal da Paraíba, Cidade Universitária, Jo?o Pessoa, PB, CEP 58059-900, Brazil 5. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil 2. Laboratório de Vacinologia, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil 3. Laboratório de Imunologia Aplicada, Núcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, 96010-900, Brazil |
| Abstract: | The anti-tumor effects of a newly-discovered lectin, isolated from okra, Abelmoschus esculentus (AEL), were investigated in human breast cancer (MCF7) and skin fibroblast (CCD-1059 sk) cells. AEL induced significant cell growth inhibition (63 %) in MCF7 cells. The expression of pro-apoptotic caspase-3, caspase-9, and p21 genes was increased in MCF7 cells treated with AEL, compared to those treated with controls. In addition, AEL treatment increased the Bax/Bcl-2 ratio in MCF7 cells. Flow cytometry also indicated that cell death (72 %) predominantly occurred through apoptosis. Thus, AEL in its native form promotes selective antitumor effects in human breast cancer cells and may represent a potential therapeutic to combat human breast cancer. |
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