Microbial production of 2,3-butanediol by a newly-isolated strain of Serratia marcescens |
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Authors: | Litao Shi Songsong Gao Yue Yu Hongjiang Yang |
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Affiliation: | 1. Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China
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Abstract: | A newly-isolated strain of Serratia marcescens, G12, was characterized for 2,3-butanediol (2,3-BD) production. In shake-flask and batch fermentations, 2,3-BD reached 48.5 and 51 g l?1, respectively. Low amounts of (~8 g l?1) of acetoin were also formed. In fed-batch fermentations, strain G12 produced 72.8 g 2,3-BD l?1 with glucose initially at 130 g l?1. When aeration rate was increased to 2.5 vvm for the fermentation process, 2,3-BD reached 87.8 g l?1 and the highest productivity was 1.6 g l?1 h?1. Acetoin was at 6.2 g l?1. G12 therefore may be a suitable candidate strain for large-scale production of 2,3-BD. |
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