Microbial production of 2,3-butanediol by a newly-isolated strain of Serratia marcescens

A newly-isolated strain of Serratia marcescens, G12, was characterized for 2,3-butanediol (2,3-BD) production. In shake-flask and batch fermentations, 2,3-BD reached 48.5 and 51 g l^?1, respectively. Low amounts of (~8 g l^?1) of acetoin were also formed. In fed-batch fermentations, strain G12 produced 72.8 g 2,3-BD l^?1 with glucose initially at 130 g l^?1. When aeration rate was increased to 2.5 vvm for the fermentation process, 2,3-BD reached 87.8 g l^?1 and the highest productivity was 1.6 g l^?1 h^?1. Acetoin was at 6.2 g l^?1. G12 therefore may be a suitable candidate strain for large-scale production of 2,3-BD.