Cloning and nucleotide sequence of the chimpanzee c-myc gene |
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Authors: | C Argaut M Rigolet M E Eladari F Galibert |
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Affiliation: | Laboratoire de Recombinaisons Génétiques, UPR 41 CNRS, Centre Hayem, H?pital Saint-Louis, Paris, France. |
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Abstract: | A DNA fragment covering the chimpanzee c-myc locus was cloned from the DNA of peripheral blood lymphocytes, sequenced, and compared to its human c-myc counterpart. The two nucleotide sequences were found to be highly homologous (99%). The divergence rate between the two species was 0.4% in exons and 1.7% in introns. The different TATA-boxes described in the human myc gene were also identified in the chimpanzee sequence and an open reading frame (ORF) was observed which overlaps the chimpanzee c-myc first exon. This latter ORF contained three silent mutations with regard to the human region, whereas the chimpanzee Myc oncoprotein coded by exons 2 and 3 differed by two amino acids from the human one. |
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