Characterization of genetic diversity ofFrankia strains in nodules ofAlnus nepalensis (D. Don) from the Hengduan Mountains on the basis of PCR-RFLP analysis of thenifD-nifK IGS |
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Authors: | Y M Dai X Y He C G Zhang Z Z Zhang |
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Institution: | (1) Institute of Applied Ecology, Chinese Academy of Sciences, 110016 Shenyang, China;(2) Graduate School of the Chinese Academy of Sciences, 100039 Beijing, China |
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Abstract: | Nodule samples from 90A. nepalensis individuals were collected at five sites in the Hengduan Mountains. PCR-RFLP analysis of IGS betweennifD andnifK genes was directly applied to unculturedFrankia strains in the nodules. Sizes of thenifD-nifK IGS amplicons and genetic distance between the RFLP patterns from these samples were noticeably different, indicating significant
genetic variation in theFrankia population. There were some nodule samples, which produced more than one PCR fragment, and compound RFLP patterns, indicating
thatFrankia strains with different PCR-RFLP patterns coexisted in the same host plant under natural conditions. Among the 29 restriction
patterns obtained, 5 patterns were found in more than one population and occurred in the majority of samples, while each of
the other 24 patterns were represented by only one or two samples and were endemic to a particular population. From the calculatedGst and UPGMA cluster analysis, genetic diversity ofFrankia strains was inferred to be related to climate and glaciation history in the Hengduan Mountains. |
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Keywords: | Alnus nepalensis Frankia genetic diversity nifD-nifK IGS PCR-RFLP |
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