The 3′-untranslated region of brome mosaic virus RNA does not enhancetranslation of capped mRNAs in vitro

The translation enhancing ability of cis-acting 3′-terminal untranslated region (3′-UTR) of brome mosaic virus (BMV) was examined. Two chimeric mRNA constructs translated in rabbit reticulocyte lysates contained the BMV coat protein (CP) gene and NPTI gene, respectively. It was shown that the 3′-UTR of BMV RNA enhanced the translational efficiency of uncapped but not capped messages.