| Abstract: | Acidophilic and alkalophilic cadmium-resistant bacterial strains were isolated from the metal-contaminated soil of Panki thermal power plant, Kanpur, India, and two strains, namely SB21 (acidophilic) and SB20 (alkalophilic), were selected as representative strains on the basis of their high resistance to cadmium as compared to other strains. The phylogenetic analysis of the strains using 16s rDNA sequence revealed the strains to be Pseudomonas putida and Comamonas species strain, respectively. Furthermore, to determine the mechanism involved in cadmium resistance, a czc gene was amplified from the strains and sequenced. Homology of the sequences of the two strains, when compared with the available database using a BLASTn search, showed that the 650bp amplicons possess a partial czcA gene sequence. Moreover, the mechanism was confirmed by the results of atomic absorption spectroscopy and transmission electron microscopy studies, which supported the efflux mechanism in the alkalophilic strain SB20, but the acidophilic strain SB21 showed intracellular and periplasmic accumulation of metal in the cells in spite of the presence of the czcA gene, indicating the presence of multiple mechanisms of metal resistance in strain SB21. Further, the strains were characterized functionally for their bioremediation potential in cadmium-contaminated soil under acidic and alkaline conditions by performing an in-situ greenhouse experiment using mungbean plants. A marked increase in agronomical parameters was observed under acidic conditions in the presence of strain SB21. Moreover, the concentration of metal decreased in both plants and soil in the presence of these bioinoculants; however, strain SB21 was found to be a better decontaminator than SB20 and thus can further be employed in bioremediation applications. |
