Toxoplasma gondii: Electron microscopic study on the dye test reaction |
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Authors: | Takuro Endo Akio Kobayashi |
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Affiliation: | 1. Department of Parasitology, National Institute of Health, Tokyo 141, Japan;2. Department of Parasitology, The Jikei University School of Medicine, Tokyo 105, Japan |
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Abstract: | When intact Toxoplasma trophozoites were stained with isotonic alkaline methylene blue solution, the organelles rich in nucleic acid, i.e., nucleus, free and membrane-bound ribosomes appeared as electron dense areas. When the parasites were incubated with the anti-Toxoplasma antibody and the accessory factor, swelling of the surface membrane occurred first, followed by destruction of the inner structures. In the dye test positive parasites, there were no definite organelles recognizable, as there were in the intact parasites. By the negative staining method, holes (defects) with dark central portions were observed on the surface of the parasites treated with the antibody and the accessory factor, the diameter of the holes measuring about 10–11 nm. These holes, which tended to occur in clusters, were each surrounded by a clear ring. |
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Keywords: | Parasitic protozoan Sabin-Feldman dye test Immune cytolysis Electron microscopy Negative staining Complement-induced hole Surface membrane Accessory factor Complement Methylene blue Nucleic acid |
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