Extracellular calcium and the organization of tight junctions in pancreatic acinar cells |
| |
Authors: | P Galli A Brenna P De Camilli J Meldolesi |
| |
Institution: | CNR Center of Cytopharmacology and Department of Pharmacology. University of Milan, 20129 Milan, Italy |
| |
Abstract: | In pancreatic lobules incubated in Ca2+-free Krebs-Ringer bicarbonate solution +0.5 mM EGTA tight junctions are first disarrayed and then break up into fasciae occludentes and small fibrillar fragments, which move laterally in the plane of the plasmalemma and often wind up around the gap junctions. The interruption of the continuity of tight junctions results in the disappearance of the difference in intramembranous particle density between the lateral and luminal regions of the plasmalemma. These results are consistent with the interpretation of tight junctions as dynamic structures, probably resulting from a specific polymerization of intramembranous particles and confirm that tight junctions might have a role in establishing and maintaining the regional differences of the plasmalemma. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |