Fasciola hepatica: Histochemical observations on juveniles and adults and the cytopathological changes induced in infected mouse liver |
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Authors: | M.N. Moore D.W. Halton |
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Affiliation: | Department of Zoology, The Queens University of Belfast, Belfast BT7 1NN, Northern Ireland |
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Abstract: | The histochemical characteristics of juvenile intrahepatic forms of Fasciola hepatica have been compared with those of the adults. The histochemical distributions of carboxylesterase (EC 3.1.1.1), acetylcholinesterase (EC 3.1.1.7) and alkaline phosphatase (EC 3.1.3.1) were similar in both juvenile and adult forms. Differences were apparent in occurrence and distribution of β-glucuronidase (EC 3.2.1.31) and N-acetyl-β-glucosaminidase (EC 3.2.1.29) activities in the tegument, parenchyma, and caecal cells. These may reflect the dietary mode and relationship to the host of the juvenile intrahepatic and adult bile-duct forms. Starvation of both juveniles and adults is accompanied by an increase in the granule-associated staining reactions for acid hydrolases in some of the parenchymal cells with a concomitant decrease in staining for glycogen in these cells. This response to starvation is reversible with refeeding, indicating that it is probably a genuine response to nutrient stress and not to degeneration induced by the in vitro conditions of the flukes. Carboxylesterase and acid phosphatase (EC 3.1.3.2) have been demonstrated to be polymorphic, using polyacrylamide disc electrophoresis. Changes were observed in the staining patterns with starvation. The juvenile flukes stimulated a considerable increase in the level of lysosomal hydrolases and alkaline phosphatase in mouse hepatic cells. |
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Keywords: | enzyme histochemistry lysosomal acid hydrolases infected mouse livers β-galactosidase EC 3.2.1.23 β-glucuronidase EC 3.2.1.31 carboxylesterase EC 3.1.1.1 acetylcholinesterase EC 3.1.1.7 alkaline phosphatase EC 3.1.3.1 acid phosphatase EC 3.1.3.2 |
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