STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution |
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| Authors: | Friedrich Mike Gan Qiang Ermolayev Vladimir Harms Gregory S |
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| Institution: | †Molecular Microscopy Group, Bio-Imaging Center, Rudolf Virchow Center, University of Würzburg, Würzburg, Germany;‡Department of Biology and Physics, Wilkes University, Wilkes-Barre, Pennsylvania |
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| Abstract: | We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebrafish embryos. The integrated STED-SPIM method combines the advantages of SPIM with the resolution enhancement of STED, and thus provides a method for fast, high-resolution imaging with >100 μm deep penetration into biological tissue. |
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