Impact of heparan sulfate chains and sulfur-mediated bonds on the mechanical properties of bovine lens capsule |
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| Authors: | Dyksterhuis L B Dyksterhuis L D White J F Hickey M Kirby N Mudie S Hawley A Vashi A Nigro J Werkmeister J A Ramshaw J A M |
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| Institution: | †CSIRO Materials Science and Engineering, Clayton, Australia;‡School of Biochemistry and Molecular Biology, Monash University, Clayton, Australia;§Department of Anatomy and Developmental Biology, Monash University, Clayton, Australia;¶Australian Synchrotron, Clayton, Australia |
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| Abstract: | We assessed the importance of glycosaminoglycans and sulfur-mediated bonds for the mechanical properties of lens capsules by comparing the stress-strain responses from control and treated pairs of bovine source. No significant change in mechanical properties was observed upon reduction of disulfide bonds. However, removal of glycosaminoglycan chains resulted in a significantly stiffer lens capsule, whereas high concentrations of reducing agent, which is expected to reduce the recently reported sulfilimine bond of collagen IV, resulted in a significantly less stiff lens capsule. A comparison of the diffraction patterns of the control and strongly reduced lens capsules indicated structural rearrangements on a nanometer scale. |
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