Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding |
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| Authors: | N. Harris J. E. Taylor J. A. Roberts |
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| Affiliation: | (1) Department of Physiology and Environmental Science, Faculty of Agricultural and Food Sciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, Leics, UK;(2) Present address: Division of Biological Sciences, Institute of Enviromental and Biological Sciences, Lancaster University, LA1 4YQ Lancaster, UK |
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| Abstract: | A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family. |
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| Keywords: | Lycopersicon esculentum tomato nucleoside diphosphate kinase wounding ethylene |
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