Optimisation of the degenerate oligonucleotide primed PCR (DOP-PCR) for capillary thermocycler |
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| Authors: | Kiss Csaba Kost-Alimova Maria Klein George Szekely Laszlo |
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| Affiliation: | Microbiology and Tumor Biology Center (MTC), Karolinska Institute, Nobels vag 16, S-17177 Stockholm, Sweden. csaba.kiss@mtc.ki.se |
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| Abstract: | DOP-PCR was optimised for capillary thermocycler. Five primers with five or six nucleotide anchor sequences and different restriction enzyme recognition sites were tested. The efficiency of the amplification was confirmed by gel electrophoresis and fluorescence in situ hybridisation on metaphase chromosomes. |
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