| 低氧对肺内小动脉平滑肌细胞血管紧张素转化酶活性和基因表达的影响 |
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| 作者姓名： | Yu TZ Ma CT |
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| 作者单位： | 1. 湖南中医学院药学分院药理教研室,长沙,410011
2. 湖南医科大学心血管生理研究室,长沙,410078 |
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| 摘 要： | 实验采用分离培养兔肺内小动脉平滑细胞,观察低氧对ＰＡＳＭＣ的血管紧张素转化酶活性和基因表达的影响。
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| 关 键 词： | 低氧 肺动脉 平滑肌细胞 ACE 活性 基因表达 |
| 修稿时间： | 1997年11月18 |
Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells |
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| Yu TZ,Ma CT.Effect of hypoxia on angiotensin-converting enzyme activity and gene expression of intrapulmonary artery smooth muscle cells[J].Acta Physiologica Sinica,1998,50(6):623-628. |
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| Authors: | Yu T Z Ma C T |
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| Institution: | Laboratory of Cardiovascular Physiology, Hunan Medical University, Changsha 410078. |
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| Abstract: | In other to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The main results are as follows: (1) under normoxia both ACE activity and gene expression could be detected in PASMC; and (2) the expression level of ACE mRNA, after an initial latent period of 6 h hypoxia, began to show a marked increase with time in the next 12, 24 and 48 h. Exposure to hypoxia for 24 h resulted in an increase in either culture medium or cell ACE activity, as compared with that under normoxia. The results suggest that PASMC ACE increased by hypoxia might play an important role in the development of hypoxic pulmonary hypertension. |
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| Keywords: | hypoxia pulmonary artery vascular smooth muscle cell angiotension converting enzyme (ACE) rabbit |
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