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Complete Nucleotide Sequences of 93-kb and 3.3-kb Plasmids of an Enterohemorrhagic Escherichia coli O157:H7 Derived from Sakai Outbreak |
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| Authors: | Makino Kozo; Ishii Kazuo; Yasunaga Teruo; Hattori Masahiro; Yokoyama Katsushi; Yutsudo Chikako H; Kubota Yoshino; Yamaichi Yoshiharu; Iida Tetsuya; Yamamoto Koichiro; Honda Takeshi; Han Chang-Gyun; Ohtsubo Eiichi; Kasamatsu Masaaki; Hayashi Tetsuya; Kuhara Satoru; Shinagawa Hideo |
| Institution: | 1Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University Suita, Osaka 565, Japan 2Human Genome Center, Institute of Medical Science, The University of Tokyo Shirokanedai, Minato-ku, Tokyo 108, Japan 3Genome Information Research Center, Osaka University Suita, Osaka 565, Japan 4Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University Osaka 565, Japan 5Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan 6Department of Bacteriology, School of Medicine, Shinshu University Matsumoto 390, Japan 7Graduate School of Genetic Resources Technology, Kyusyu University Hakozaki, Fukuoka 812, Japan |
| Abstract: | Enterohemorrhagic Escherichia coli (EHEC) O157:H7, derived froman outbreak in Sakai city, Japan in 1996, possesses two kindsof plasmids: a 93-kb plasmid termed pO157, found in clinicalEHEC isolates world-wide and a 3.3-kb plasmid termed pOSAK1,prevalent in EHEC strains isolated in Japan. Complete nucleotidesequences of both plasmids have been determined, and the putativefunctions of the encoded proteins and the cis-acting DNA sequenceshave been analyzed. pO157 shares strikingly similar genes andDNA sequences with F-factor and the transmissible drug-resistantplasmid R100 for DNA replication, copy number control, plasmidsegregation, conjugative functions and stable maintenance inthe host, although it is defective in DNA transfer by conjugationdue to the truncation and deletion of the required genes andDNA sequences. In addition, it encodes several proteins implicatedin EHEC pathogenicity such as an EHEC hemolysin (HlyA), a catalase-peroxidase(KatP), a serine protease (EspP) and type II secretion system.pOSAK1 possesses a ColE1-like replication system, and the DNAsequence is extremely similar to that of a drug-resistant plasmid,NTP16, derived from Salmonella typhimurium except that it lacksdrug resistance transposons. |
| Keywords: | Enterohemorrhagic Escherichia coli F-factor nucleotide sequence pathogenicity plasmid pO157 pOSAK1 |
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