Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos |
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Authors: | Sommer Jeffrey R Collins E Bruce Estrada Jose L Petters Robert M |
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Institution: | Department of Animal Science, North Carolina State University, Campus Box 7621, Raleigh, NC 27695-7621, USA. |
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Abstract: | An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse, PG600 and Chorulon along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal. |
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Keywords: | Gilt Superovulation Estrous synchronization Pronuclear stage embryo PG600® Prostaglandin F2α Transgenic |
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