蛋白质磷酸化修饰的研究进展

蛋白质磷酸化是最常见、最重要的一种蛋白质翻译后修饰方式,它参与和调控生物体内的许多生命活动。通过蛋白质的磷酸化与去磷酸化,调控信号转导、基因表达、细胞周期等诸多细胞过程。随着蛋白质组学技术的发展和应用,蛋白质磷酸化的研究越来越受到广泛的重视。我们介绍了蛋白质磷酸化修饰的主要类型与功能、磷酸化蛋白质分析样品的富集及制备、磷酸化蛋白的鉴定及磷酸化位点的预测、蛋白分离后磷酸化蛋白的检测,及蛋白质磷酸化的分子机制,并综述了近年来国内外的主要相关研究进展。

Progress on Protein/Peptide Phosphorylation

Phosphorylation is one of the most important post-translational modifications of proteins, which is related to many activities of life. By reversible protein phosphorylation eukaryotes control many cellular processes including signal transduction, gene expression, and the cell cycle etc. As the development and application of the proteomics, the studies of the protein phosphorylation have become more important. This article has introduced the main types and functions of the protein phosphorylation, the enrichment and preparation of phosphoproteins and phosphopeptides, the identification of the phosphopeptides, the determination and prediction of the specifie-phosphorylation-site, the phosphorelated modifications of the proteins, and the progress on studies above as well.