The kanamycin resistance transposon Tn2680 derived from the R plasmid Rts1 and carried by phage P1Km has flanking 0.8-kb-long direct repeats |
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| Authors: | Shigeru Iida Jürg Meyer Patrick Linder Nobuichi Goto Rintaro Nakaya Hans-Jörg Reif Werner Arber |
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| Affiliation: | 2. Department of Microbiology, Biozentrum of the University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;1. Department of Microbiology, Tokyo Medical and Dental University, School of Medicine, Yushima 1-5-45, Bunkyo, Ku, Tokyo 113, Japan |
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| Abstract: | Phage P1Km carries within the invertible DNA segment a 5-kb insertion with 0.8-kb terminal direct repeats flanking the kanamycin resistance determinant. The same structure was also found on the R plasmid Rts1, from which the Km resistance segment of P1Km was derived. Obviously, this Km resistance segment translocated as a unit to the P1 genome and it is therefore called Tn2680. Loss of the Km resistance determinant due to recombination between the flanking direct repeats occurs during vegetative growth of P1Km. Amplification of Tn2680 to tandem oligomers is documented and is thought to result from recombination between the flanking direct repeats. The flanking 0.8-kb repeats are different from known IS elements. |
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| Keywords: | To whom correspondence should be addressed. |
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