Contaminant loading in remote Arctic lakes affects cellular stress-related proteins expression in feral charr |
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Authors: | Steve Wiseman Even H J?rgensen Alec G Maule Mathilakath M Vijayan |
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Institution: | (1) Department of Biology, University of Waterloo, Waterloo, ON, N2L 3G1, Canada;(2) Norwegian College of Fishery Science, University of Troms?, Troms?, Norway;(3) USGS, Cook, WA, USA; |
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Abstract: | The remote Arctic lakes on Bj?rn?ya Island, Norway, offer a unique opportunity to study possible affect of lifelong contaminant
exposure in wild populations of landlocked Arctic charr (Salvelinus alpinus). This is because Lake Ellasj?en has persistent organic pollutant (POP) levels that are significantly greater than in the
nearby Lake ?yangen. We examined whether this differential contaminant loading was reflected in the expression of protein
markers of exposure and effect in the native fish. We assessed the expressions of cellular stress markers, including cytochrome
P4501A (Cyp1A), heat shock protein 70 (hsp70), and glucocorticoid receptor (GR) in feral charr from the two lakes. The average
polychlorinated biphenyl (PCB) load in the charr liver from Ellasj?en was approximately 25-fold higher than in individuals
from ?yangen. Liver Cyp1A protein expression was significantly higher in individuals from Ellasj?en compared with ?yangen,
confirming differential PCB exposure. There was no significant difference in hsp70 protein expression in charr liver between
the two lakes. However, brain hsp70 protein expression was significantly elevated in charr from Ellasj?en compared with ?yangen.
Also, liver GR protein expression was significantly higher in the Ellasj?en charr compared with ?yangen charr. Taken together,
our results suggest changes to cellular stress-related protein expression as a possible adaptation to chronic-contaminant
exposure in feral charr in the Norwegian high-Arctic. |
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