Characterization of restriction endonuclease AjoI from Acinetobacter johnsonii |
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| Authors: | A Nowak J Kur E Gospodarek K Bielawski |
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| Institution: | Department of Microbiology, Technical University of Gdańsk, ul. Gabriela Narutowicza 11/12, 80-952 Gdańsk, Poland; Medical Academy of Bydgoszcz, Department of Microbiology, ul. M. Skłodowska-Curie 9, 85-094 Bydgoszcz, Poland; Department of Molecular Biology, University of Gdańsk, ul. Kładki 24, 80-822 Gdańsk, Poland |
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| Abstract: | Abstract A new type II restriction endonuclease, named Ajo I, was detected in Acinetobacter johnsonii . The enzyme Ajo I, an isoschizomer of Pst I, recognized the hexanucleotide sequence 5'-CTGCA/G-3'], with a cleavage site generating fragments of DNA with protruding cohesive 3' termini. |
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| Keywords: | Acinetobacter johnsonii Restriction endonuclease Isoschizomer Recognition sequence PstI endonuclease |
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