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Morphogenetic movements during axolotl neural tube formation tracked by digital imaging |
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| Authors: | G. Wayne Brodland Michael J. Scott Andrew F. MacLean M. Globus S. Vethamany-Globus R. Gordon Jim H. Veldhuis R. Del Maestro |
| Affiliation: | (1) Department of Civil Engineering, University of Waterloo, N2L 3G1 Waterloo, ON, Canada;(2) Department of Medical Biophysics, University of Western Ontario, N6A 3K7 London, ON, Canada;(3) Department of Biology, University of Waterloo, N2L 3G1 Waterloo, ON, Canada;(4) Department of Radiology, University of Manitoba, R3A 1R9 Winnipeg, MB, USA;(5) Brain Research Laboratories, Experimental Research Unit, Victoria Hospital Research Institute, N6A 4G5 London, ON, Canada |
| Abstract: | During neurulation in vertebrate embryos, epithelial cells of the neural plate undergo complex morphogenetic movements that culminate in rolling of the plate into a tube. Resolution of the determinants of this process requires an understanding of the precise movements of cells within the epithelial sheet. A computer algorithm that allows automated tracking of epithelial cells visible in digitized video images is presented. It is used to quantify the displacement field associated with morphogenetic movements in the axolotl (Ambystoma mexicanum) neural plate during normal neural tube formation. Movements from lateral to medial, axial elongations and area changes are calculated from the displacement field data and plotted as functions of time. Regional and temporal differences are identified. The approach presented is suitable for analyzing a wide variety of morphogenetic movements. |
| Keywords: | Cell tracking Morphogenetic movements Image processing Neurulation Axolotl |
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