Production of wheat-rye substitution lines based on winter rye cultivars with karyotype identification by means of C-banding, GISH, and SSR markers

The study presents a continuation of the research aimed at producing of wheat-rye substitution lines based on the cross (Triticum aestivum L. x Secale sereale L.) x Triticum aestivum L., and using winter rye cultivars Vyatka and Vietnamskaya Mestnaya. In BC1F5 two lines were identified, having karyotypes in which a pair of homologous wheat chromosomes was substituted by a homeologous pair of rye chromosomes. The chromosome composition of these lines was analyzed using C-banding, GISH, and SSR markers. It was demonstrated that karyotype of each line included a single pair of rye chromosomes and lacked wheat-rye translocations. The rye chromosomes were identified, and the chromosomes of wheat, at which the substitutions occurred, were determined. The lines generated by crosses with rye of Vyatka and Vietnamskaya Mestnaya cultivars were designated 1Rv(1A) and 5Rviet(5A), respectively. Chromosome identification and classification of the lines makes it possible to use them in breeding programs and genetic studies.