三种蚤生殖系统发育的细微结构:雌性生殖腺的发育

本文研究的缓慢细蚤Leptopsylla segnis (Schonherr,1811),不等单蚤Monopsyllus anisus (Rotbschild,1907)和猫栉首蚤指名亚种Ctenocephalides felis felis (Bouchè,1835)均采自贵阳市区,鼠、猫宿主体上。通过饲养与繁殖,制作从三龄幼虫至成虫成熟各发育时间的连续切片。预察了各发育时间生殖腺发育的内部结构变化,依据卵巢芽的发育、分化,末端最大卵母细胞形态和大小的变化,卵泡上皮细胞形态和大小的变化,卵泡间组织的出现,卵黄的产生、积累及卵壳物质的分泌等,将雌性生殖腺的发育分为四期10个阶段,并对雌性生殖腺发育内部结构的变化,雌蚤卵黄沉淀初期的“空位现象”等进行了分析析和讨论。

FINE STRUCTURES OF THE REPRODUCTIVE SYSTEM OF THREE FLEA SPECIES: DEVELOPMENT OF FEMALE GONADS

A study on the internal structures during the development and maturation of the female gonads from the third instar larva up to mature adult of Leptopsylla segnis (Schon-herr, 1811), Monopsyllus anisus (Rothschild, 1907) and Ctenocephdides felis felis (Bou-che, 1835) is reported in this paper.All the specimens were collected from Guiyang and reared in our laboratory. The serial sections of every developmental stage from the third instar larva through pupa to mature adult were made between certain intervals by a modified Rothschild's (1975) technique. They were stained with modified Mallory's triple stain, HE stain and treated with PAS reaction.Results of these studies are summarized as following:(1)The development of the female gonads of these fleas is essentially the same. How ever, there are differences in the number of ovarioles and in the time required for development and maturation of oocytes.(2)According to the development of the ovarial rudiments, the degree of development of oocytes and follicular epithelium, the quantity of accumulated yolk and the appearance of chorion etc., the development and maturation process of the female gonads from the third instar larva up to mature adult could be divided into 4 phases with 10 stages.(3)The 'open spaces' between follicular cells were observed when vitellogenesis began at the 8th stage during the development of female gonads. It was presumed that the vi-tellogenin molecules which were formed in the fat body after entering into hemolymph, could pass through 'open spaces' between the follicular cells and the membrane of oocytes to form the yolk granules.