Normal specification of the extraembryonic lineage after somatic nuclear transfer |
| |
Authors: | Kishigami Satoshi Hikichi Takafusa Van Thuan Nguyen Ohta Hiroshi Wakayama Sayaka Bui Hong-Thuy Mizutani Eiji Wakayama Teruhiko |
| |
Institution: | Laboratory for Genomic Reprogramming, Center for Developmental Biology RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047, Japan. kiskigami@cdb.riken.jp |
| |
Abstract: | To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressing blastocysts were as low as 50% and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained. |
| |
Keywords: | Mouse Nuclear transfer Preimplantation Cdx2 Oct4 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|