Immunochemical quantification of cytochrome f in leaves of a non-yellowing senescence mutant of Festuca pratensis |
| |
Authors: | T G Emyr Davies Howard Thomas Lyndon J Rogers |
| |
Institution: | (1) Welsh Plant Breeding Station, Cell Biology Department, AFRC Institute for Grassland and Animal Production, Plas Gogerddan, SY23 3EB Aberystwyth, Dyfed, UK;(2) Department of Biochemistry, University College of Wales, SY23 3DD Aberystwyth, Dyfed, UK |
| |
Abstract: | A non-competitive enzyme-linked immunosorbent assay (ELISA) which enables detection of as little as 0.1 ng cytochrome f in leaf extracts has been developed. No evidence for specific or non-specific interference by proteins other than cytochrome f was found. The assay was applied to a comparative study of age-related changes in the cytochrome f content of leaves of Festuca pratensis Huds. cv. Rossa, and a non-yellowing mutant genotype (Bf993) having a lesion in the mechanism responsible for thylakoid membrane disassembly. Cytochrome f in senescent leaves of the latter genotype was found to be present at significantly higher levels than in the wild-type, implying an inability on the part of the mutant to degrade this protein. The results obtained by ELISA were confirmed by antibody probing of Western blots.Abbreviations Ab
antibody
- BSA
bovine serum albumin
- chl
chlorophyll
- cyt
cytochrome
- ELISA
enzyme-linked immunosorbent assay
- Ig
immunoglobulin
- kDa
kilodalton
- LHCP-2
light-harvesting chlorophyll a/b binding protein
- OEC
oxygen-evolving complex
- PAGE
polyacrylamide gel electrophoresis
- POase
peroxidase
- SDS
sodium dodecyl sulphate
- PS II
Photosystem II
- TBS
Tris buffered saline
- Tris
tris (hydroxymethyl) aminomethane |
| |
Keywords: | cytochrome f ELISA Festuca pratensis senescence |
本文献已被 SpringerLink 等数据库收录! |
|