Structured PDMS Chambers for Enhanced Human Neuronal Cell Activity on MEA Platforms |
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| Authors: | Joose Kreutzer Laura Ylä-Outinen Paula Kärnä Tiina Kaarela Jarno Mikkonen Heli Skottman Susanna Narkilahti Pasi Kallio |
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| Institution: | 1. Department of Automation Science and Engineering Tampere University of Technology Korkeakoulunkatu 3, 33720 Tampere, Finland;2. BioMediTech Biokatu 10, 33520 Tampere Finland;3. NeuroGroup, Institute of Biomedical Technology, University of Tampere, Biokatu 12, 33520 Tampere, Finland;4. The Science Center of Pirkanmaa Hospital District, Biokatu 12, 33521 Tampere, Finland;5. Department of Psychology, University of Jyväskylä, Ylistönmäentie 33, 40500 Jyväskylä, Finland |
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| Abstract: | Structured poly(dimethylsiloxane) (PDMS) chambers were designed and fabricated to enhance the signaling of human Embryonic Stem Cell (hESC) - derived neuronal networks on Microelectrode Array (MEA) platforms.The structured PDMS chambers enable cell seeding on restricted areas and thus,reduce the amount of needed coating materials and cells.In addition,the neuronal cells formed spontaneously active networks faster in the structured PDMS chambers than that in control chainbers.In the PDMS chambers,the neuronal networks were more active and able to develop their signaling into organized signal trains faster than control cultures.The PDMS chamber design enables much more repeatable analysis and rapid growth of functional neuronal network in vitro.Moreover,due to its easy and cheap fabrication process,new configurations can be easily fabricated based on investigator requirements. |
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