Characterization of monoclonal antibodies against human parvovirus B19 |
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Authors: | N Yaegashi K Tada H Shiraishi T Ishii K Nagata K Sugamura |
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Affiliation: | Department of Microbiology, Tohoku University School of Medicine, Miyagi. |
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Abstract: | Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions. |
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