Isolation of DNA-dependent RNA polymerase from the thermophilic actinomyceteThermomonospora curvata |
| |
| Authors: | M Krausová M Klégr J Spížek |
| |
| Institution: | (1) Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4 |
| |
| Abstract: | DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated fromThermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation
with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5m. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
