Application of PCR for Detection of Mycoplasma DNA and Pestivirus RNA in Human Live Viral Vaccines |
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| Authors: | Tsuguo Sasaki Ryo Harasawa Miharu Shintani Hiroshi Fujiwara Yuko Sasaki Atsuko Hirino Tsuyoshi Kenri Kiyozo Asada Ikunoshin Kato Fumitoshi Chino |
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| Affiliation: | aDepartment of Safety Research on Biologics, National Institute of Health, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208, Japan;bAnimal Center for Biomedical Research, Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;cBiotechnology Research Laboratories, Takara Shuzo Co., Ltd. Otsu-shi, Shiga 520-21, Japan |
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| Abstract: | PCR techniques were applied for the detection of mycoplasma DNA and pestivirus RNA to 43 lots of live viral vaccines (measles, mumps, rubella, and oral poliomyelitis) produced by six manufacturers in Japan. Although mycoplasma DNA was not detected in any of the vaccines tested, pestivirus RNA was detected in 12 lots (28%). The incidence of contamination among the four viral vaccines was in the range of 20 to 37%, and the incidence among the six manufacturers varied from 0 to 56%. |
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