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| 组织培养创造抗白粉病小麦-簇毛麦染色体易位及分子标记辅助选择 | |
| 引用本文： | 李洪杰,李义文,贾旭,李洪杰,张艳敏,李辉,郭北海,GUO Bei-Hai,王子宁,温之雨,朱至清,刘志勇.组织培养创造抗白粉病小麦-簇毛麦染色体易位及分子标记辅助选择[J].遗传学报,2000,27(7):608-613. |
| 作者姓名： | 李洪杰 李义文 贾旭 李洪杰 张艳敏 李辉 郭北海 GUO Bei-Hai 王子宁 温之雨 朱至清 刘志勇 |
| 作者单位： | 1. 中国科学院遗传研究所植物细胞与染色体工程国家重点实验室，北京 100101 2. 河北省农林科学院粮油作物研究所河北省作物遗传育种实验室，石家庄 050031 3. Hebei Provincial Laboratory of Crop Genetics and Breeding, Institute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050031, China 4. 中国农业大学植物科学院，北京 100094 |
| 基金项目： | 中国科学院重大项目；河北省博士资金项目资助 |
| 摘 要： | 对175株普通小麦与6D/6V代换系杂种当代幼胚培养再生植株进行谷草转氨酶同工酶分析，其中来自杂交组合遗4095×RW15（6D／6V代换系）的2个植株（编号分别为98R149和98R159）GOT—V |
| 关 键 词： | 组织培养 小麦 白粉病 染色体易位 抗病育种 |
| 文章编号： | 0379-172（2000）07-608-6 |
| 修稿时间： | 1999年8月25日 |
Tissue Culture Induced Translocation Conferring Powdery Mildew Resistance Between Wheat and Dasypyrum villosum and Its Marker-assisted Selection |
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| GUO Bei-Hai.Tissue Culture Induced Translocation Conferring Powdery Mildew Resistance Between Wheat and Dasypyrum villosum and Its Marker-assisted Selection[J].Journal of Genetics and Genomics,2000,27(7):608-613. | |
| Authors: | GUO Bei-Hai |
| Abstract: | Glutamate oxaloacetate transaminase (GOT)electrophoretic analyses were Performed in 175 regenerants arising from immature embryos of crosses between wheat (Triticum aestivum L.) and 6D / 6V substitution stocks. The GOT-V2, coding specific enzyme band was absent in two regenerants, designated 98R149 and 98R159 respectively, originated from cross of Yi 4095 and 6D / 6V substitution stick c.v. RW15. Pm21 gene linked SCARs (Sequence Characteried Amplifled Regions) analysis indicated that 6VS chromosome arms existed in 98R149 and 98R159. Fluorescence in situ hybridization with total genomic DNA extrdeted from Dasypyrum villosum (L) as a probe confirmed the occurrence of translocation between 6V chromosome and a wheat one in the two regenerants mentioned above. 98R149 and 98R159 were immune to powdery mildew (Erysiphe graminisDC. f sp. tritici) inoculation with mix races collected from Hebei Province. The results of the present paper added another feasible example of useful translocations via hssue culture. |
| Keywords: | Pm21 tissue culture translocation glutamate oxaloacetate transaminase (GOT) SCAR fluorescence in situ hybrisization marker-assisted selection |
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