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Vasopressin-dependent control of basolateral Na/H-exchange in highly differentiated A6-cell monolayers |
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| Authors: | Lorenzo Guerra Valeria Casavola Sebastiane Vilella François Verrey Corinna Helmle-Kolb Heini Murer |
| Institution: | (1) Department of Physiology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland;(2) University of Bari, Institute of Physiology, Via Amendola 165/A, I-70126 Bari, Italy;(3) Department of Biology, Faculty of Science, University of Lecce, Via Prov. le Lecce Monteroni, I-73100 Lecce, Italy |
| Abstract: | We have used a well-differentiated A6-cell preparation (A6-C1) to study cellular location and vasopressin control of Na/H-exchange activity. After cell acidification, cell pHi (measured by BCECF-fluorescence) only recovered by the addition of Na medium to the basolateral cell surface; this pHi recovery was inhibited by dimethylamiloride (2  m) consistent with basolateral location of Na/H-exchange activity. Addition of vasopressin produced stimulation of Na/H-exchange activity and increased the affinity of the exchanger for Na+. Stimulation of Na/H exchange was mimicked by pharmacological activation of protein kinase A (forskolin, 8-Br-cAMP) and not by pharmacological activation of protein kinase C (TPA). It is concluded that basolaterally located Na/H-exchange in A6-C1 cells is activated by vasopressin. |
| Keywords: | Na+/H+ exchange Protein kinases cAMP Vasopressin Distal tubule Tissue culture |
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