Staining of the spindle apparatus in human lymphocyte and fibroblast cultures |
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Authors: | F. Pera K. Sperling |
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Affiliation: | (1) Anatomisches Institut der Universität Bonn, Bonn;(2) Genetisches Institut der Freien Universität Berlin, Berlin |
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Abstract: | Summary The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol-acetic acid and treated for about 15 min with 0.1xSSC at 92°C and for 18 h with 2xSSC at 60°C. |
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