Ouabain-insensitivity of highly active Na+, K+-dependent adenosinetriphosphatase from rat kidney. |
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| Authors: | S M Periyasamy L K Lane A Askari |
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| Institution: | 1. Department of Pharmacology and Therapeutics Medical College of Ohio Toledo, Ohio 43699 USA;2. Department of Pharmacology and Cell Biophysics University of Cincinnati College of Medicine Cincinnati, Ohio 45267 USA |
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| Abstract: | Highly purified preparations of Na++K+-dependent adenosinetriphosphatase were isolated from rat kidney by two different procedures. The I50 values for ouabain inhibition of the rat kidney enzyme at various stages of purification were determined to be essentially the same for all fractions tested (0.7 to 1.0 × 10?4M). These results suggest that the marked insensitivity of the rat enzyme to inhibition by cardiac glycosides is due to the primary structure of the enzyme, and not to some other component in the tissue. |
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| Keywords: | SDS sodium dodecyl sulfate |
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