Imipenem-resistant Gram-negative bacterial isolates carried by persons upon medical examination in Korea |
| |
Authors: | So Yeon Kim Sang Yop Shin Ji-Young Rhee Kwan Soo Ko |
| |
Institution: | 1.Department of Molecular Cell Biology,Sungkyunkwan University School of Medicine,Suwon,Republic of Korea;2.Korea Medical Institute,Seoul,Republic of Korea;3.Division of Infectious Diseases, Department of Medicine,Dankook University,Cheonan,Republic of Korea |
| |
Abstract: | Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method. In total, 82 IR-GNB bacterial isolates were obtained from 79 (26.3%) out of 300 healthy persons. Multilocus sequence typing analysis showed very high diversity among IR P. aeruginosa, S. maltophilia, and E. cloacae isolates, and pulsed-field gel electrophoresis revealed five main pulsotypes of IR P. mirabilis. As for the presence of metallo-β-lactamases (MBLs), only one IMP-25-producing S. marcescens isolate was identified. Although only one carbapenemase-producing isolate was identified, the high colonization rates with IR-GNB isolates in this study is notable because carriers may be a reservoir for the dissemination of resistant pathogens within the community as well as in health care institutions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|