Somatic embryogenesis and plant regeneration from barley cultivars grown in Spain |
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Authors: | A M Castillo B Egaña J M Sanz L Cistué |
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Institution: | (1) Dpto. Genética y Producción Vegetal, Estación Experimental de Aula Dei. Apdo. 202, E-50080 Zaragoza, Spain Fax: +34-76-575620 e-mail: amcast@eead.csic.es, ES |
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Abstract: | Thirty-two barley cultivars grown in Spain, 18 of the two-row type and 14 of the six-row type, were screened for plant regeneration
from cultured immature embryos. Although there was much variation in regeneration capacity among the cultivars, plants were
obtained from all cultivars except Almunia. No statistical differences were found in the percentage of regeneration between
two- and six-row types. The influence of the auxins 2,4-dichlorophenoxyacetic acid, dicamba, and picloram on the induction
and maintenance of embryogenesis and regeneration capacity after 3–4 months in culture, were evaluated for cultivars Cobra,
Hop and Reinette. Hop had the highest rates of maintenance of embryogenic capacity and plant regeneration. The medium containing
dicamba gave the best embryogenic callus induction, maintenance and regeneration. Five regeneration media, differing in growth
regulators and micronutrient composition, as well as partial desiccation of the calli before regeneration, were tested. The
regeneration medium containing 10 μm copper sulfate gave the best results. Regeneration frequencies after 3–4 months in culture of cultivar Hop were raised from
59.5 to 93.7% in this medium. Silver nitrate and partial desiccation of the calli also enhanced plant regeneration, but the
medium containing 10 μm of silver nitrate reduced root formation.
Received: 30 October 1997 / Revision received: 3 April 1998 / Accepted: 17 April 1998 |
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Keywords: | Barley Somatic embryogenesis Plant regeneration |
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