Purification of cardiac (Na+,K+)-activated adenosine triphosphatase from rat |
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| Authors: | T Watanabe Y Tawada M Shigekawa |
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| Affiliation: | Department of Molecular Physiology, National Cardiovascular Center Research Institute, Osaka, Japan. |
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| Abstract: | A procedure is described for preparation of highly active (Na+,K+)-ATPase from rat heart which has a specific activity of 200-600 mumol Pi/mg/h. The procedure is simple and can be applied to small amounts of heart muscle (approximately 1 g). The ATPase activity was more than 90% sensitive to ouabain (at concentrations up to 1 mM). The ouabain sensitivity is biphasic with about 20% of the ATPase activity being inhibited at approximately 3 X 10(-7) M ouabain. |
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