| 培养基组成对树状多节孢(Nodulisporium sylviforme)紫杉醇产量的影响 |
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| 引用本文： | 赵凯,周东坡,王伟.培养基组成对树状多节孢(Nodulisporium sylviforme)紫杉醇产量的影响[J].菌物研究,2003(1). |
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| 作者姓名： | 赵凯 周东坡 王伟 |
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| 作者单位： | 黑龙江大学生命科学学院，黑龙江大学生命科学学院，黑龙江大学生命科学学院 黑龙江哈尔滨150080，东北农业大学食品科学技术学院，黑龙江哈尔滨150030，黑龙江哈尔滨150080，黑龙江哈尔滨150080 |
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| 基金项目： | 黑龙江省“十五”重大攻关项目(GA0 2C10 1)，哈尔滨市重点项目(0 114 2 112 6) |
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| 摘 要： | 采用正交试验法 ,研究了醋酸钠、苯丙氨酸、酪氨酸、亮氨酸对紫杉醇产生菌HQD3 3 产生紫杉醇的影响。结果表明 ,它们之间的协同作用对提高紫杉醇产量有显著影响。在改良的S - 7培养基基础上 ,再加入醋酸钠 1 0g/L、苯丙氨酸 5 0mg/L、酪氨酸 1 5mg/L、亮氨酸 6 0mg/L ,可以使紫杉醇产生菌HQD3 3 产生紫杉醇产量提高到 2 0 3 6 5 6 μg/L。
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| 关 键 词： | 树状多节孢 紫杉醇产生菌 微生物发酵 紫杉醇 前体 |
Effects of Medium Components on Taxol Production of Nodulisporium sylviforme |
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| ZHAO Kai ,ZHOU Dong po ,WANG Wei Life and Science College,Heilongjiang University,Harbin,Heilongjiang ,China Food and Science Institute,Northeast Agricultural University,Harbin,Heilongjiang ,China.Effects of Medium Components on Taxol Production of Nodulisporium sylviforme[J].Journal of Fungal Research,2003(1). |
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| Authors: | ZHAO Kai ZHOU Dong po WANG Wei Life and Science College Heilongjiang University Harbin Heilongjiang China Food and Science Institute Northeast Agricultural University Harbin Heilongjiang China |
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| Institution: | ZHAO Kai 1,2,ZHOU Dong po 1,WANG Wei 1 1 Life and Science College,Heilongjiang University,Harbin,Heilongjiang 150080,China 2 Food and Science Institute,Northeast Agricultural University,Harbin,Heilongjiang 150030,China |
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| Abstract: | The effects of the components of media on taxol producing strain Nodulisporium sylviforme were studied in the experiment by using orthogonal experiment The results showed that their interactions dramatically promoted taxol biosynethesis On the basic of the primary S 7, the addition of 1 0 mg/L acetate, 5 0 mg/L phenylalanine, 1 5 mg/L tyrosine and 6 0 mg/L leucine into the media dramatically increased taxol yield to 203 65 μg/L |
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| Keywords: | Nodulisporium sylviforme HQD 33 microbiological fermentation taxol precursor |
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