NIRF在体内外可明显抑制乙型肝炎病毒抗原的表达

随着对NIRF（Np95/ICBP-90 like RING finger protein）研究的深入，其功能已涉及细胞癌变进程以及表观遗传学等领域. 近期研究显示,NIRF能与HBc (hepatitis B virus core protein )相互结合，但其对乙型肝炎病毒(HBV)抗原表达的影响尚不明确. 本文通过转染pAAV-HBV1.3质粒和高压水动力法尾静脉注射BALB/C小鼠，建立乙型肝炎病毒的细胞和动物模型，研究NIRF对乙型肝炎病毒抗原表达的影响. ELISA检测细胞上清和小鼠血清中HBsAg、HBeAg的分泌和表达情况，Western 印迹或免疫组化染色技术检测HBcAg. 结果显示，乙型肝炎病毒抗原分泌的细胞以及小动物模型建立成功，并且无论在体内外，NIRF都能对它们的表达起抑制作用，期待能为后续的HBV致病机理以及治疗药物的研究提供支持与帮助.

NIRF Can Significantly Inhibit the Expression of Hepatitis B Virus Antigens in vitro and in vivo

NIRF (Np95/ICBP-90 like RING finger protein) is a protein that has been suggested to function in carcinogenesis and epigenetic modifications from recent research. Reports have shown that NIRF interacted with the hepatitis B virus core protein (HBc),however，the effects of NIRF on the secretion of hepatitis B virus (HBV) antigens remained unclear. Here,we established both the cell culture and animal models of HBV infection by the transfection of pAAV-HBV1-3 plasmid into HepG2 cells or venous hydrodynamical injection of the plasmid in BALB/C mice to explore the connection between NIRF and HBV antigen expression in vitro and in vivo. The secretion of HBsAg and HBeAg in the cultural supernatants of the transfected cells or the sera of the injected mice were analyzed by ELISA. The HBcAg secretion was assayed by Western blot or immunohistochemical analysis. The results demonstrated that the expression and secretion of HBV antigens were decreased following NIRF overexpression both in vitro and in vivo. Our findings suggested that the inhibition of NIRF on HBV antigens might provide a better understanding of HBV infection for developing new therapeutic strategies against hepatitis B virus.